Inocula are prepared by growing C. neoformans in liquid YPAD overnight at 30 °C. Cells are counted by hemocytometer and, for an intranasal infection, 1×107 cells are washed twice with PBS and resuspended in 1 ml of PBS.
What is an inoculum in microbiology?
Definition. noun, plural. (1) Cells used in an inoculation, such as cells added to start a culture. (2) A biological material (like a virus or toxin or immune serum) that is injected into a human to induce or increase immunity to a particular disease.
What is the process of inoculation?
Inoculation, process of producing immunity and method of vaccination that consists of introduction of the infectious agent onto an abraded or absorptive skin surface instead of inserting the substance in the tissues by means of a hollow needle, as in injection.
