A trypsin digest is used to cleave the proteins in a sample downstream to every K (lysine) or R (arginine), except when followed by P (proline). The individual components that result after the cleavage step are called tryptic peptides.

What is a semi tryptic peptide?

Semi-Tryptic Peptides are peptides which were cleaved at the C-Terminal side of arginine (R) and lysine (K) by trypsin at one end but not the other. ... Higher prevalence of Semi-Typtic Peptides can indicate digestion preparation problems.

What is tryptic mapping?

TRYPTIC MAPPING : USES AND LIMITATIONS  USES : • It's one of the most demanding application of HPLC • It's the primer method for structural determination of newly discovered proteins • Use to locate glycosylation sites and disulfide linkages • Provides vital information on lot-to-lot product consistency, expression ...