Reconstitute Alpha Naphthol powder by adding 12 ml of 95% ethanol to the vial and mix well. The reconstituted product has a shelf life of 2-weeks when stored at 2-8°C. 1. Inoculate a tube of MR-VP Broth with a pure culture of the isolate to be tested and incubate at 35-37°C for 24-72 hours.
How do you make alpha naphthol for VP test?
After 6 hours of incubation at 35°C in ambient air, add 1.2 mL (12 drops) of solution A (alpha-naphthol) and 0.4 mL (4 drops) solution B (40% KOH with creatine). Shake the tube and incubate at room temperature for 30 minutes.
How is alpha naphthol prepared from naphthalene?
1-Naphthol is prepared by two main routes. In one method, naphthalene is nitrated to give 1-nitronaphthalene, which is hydrogenated to the amine followed by hydrolysis: C10H8 + HNO3 → C10H7NO2 + H2O. C10H7NO2 + 3 H2 → C10H7NH2 + 2 H2O.
