Spheroids can be grown with a few different methods. One common method is to use low cell adhesion plates, typically a 96 well plate, to mass-produce spheroid cultures, where the aggregates form in the rounded bottom of the cell plates.

What are cell spheroids?

Spheroids, the three-dimensional (3D) cell cultures that arrange themselves during proliferation into sphere-like formations, got their name in the 1970s, when scientists observed that hamster lung cells grown in suspension arranged themselves in a nearly perfect spherical form.

Why do cancer cells form spheroids?

The formation of multicellular spheroidal cell aggregates, or spheroids, is a conspicuous characteristic of cancer stem cells (CSCs) and tumor-initiating cells (TICs) that possess the ability for self-renewal, proliferation and generation of downstream progenitor cells to promote tumor growth [1].