The bacterial DNA causing disease is situated on a plasmid, Ti plasmid (Tumour inducing plasmid, 200kb) instead of on a bacterial chromosome. Thus, it is used as vector in recombination process.

What is vector in recombinant DNA technology?

A vector is any vehicle, often a virus or a plasmid that is used to ferry a desired DNA sequence into a host cell as part of a molecular cloning procedure. Depending on the purpose of the cloning procedure, the vector may assist in multiplying, isolating, or expressing the foreign DNA insert.

Why is a vector used in Rdna technology?

In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g., plasmid, cosmid, Lambda phages). A vector containing foreign DNA is termed recombinant DNA.