Using the csv module:

import csv

with open('file.csv', newline='') as f:
    reader = csv.reader(f)
    data = list(reader)

print(data)

Output:

[['This is the first line', 'Line1'], ['This is the second line', 'Line2'], ['This is the third line', 'Line3']]

If you need tuples:

import csv

with open('file.csv', newline='') as f:
    reader = csv.reader(f)
    data = [tuple(row) for row in reader]

print(data)

Output:

[('This is the first line', 'Line1'), ('This is the second line', 'Line2'), ('This is the third line', 'Line3')]

Old Python 2 answer, also using the csv module:

import csv
with open('file.csv', 'rb') as f:
    reader = csv.reader(f)
    your_list = list(reader)

print your_list
# [['This is the first line', 'Line1'],
#  ['This is the second line', 'Line2'],
#  ['This is the third line', 'Line3']]

Updated for Python 3:

import csv

with open('file.csv', newline='') as f:
    reader = csv.reader(f)
    your_list = list(reader)

print(your_list)

Output:

[['This is the first line', 'Line1'], ['This is the second line', 'Line2'], ['This is the third line', 'Line3']]

Pandas is pretty good at dealing with data. Here is one example how to use it:

import pandas as pd

# Read the CSV into a pandas data frame (df)
#   With a df you can do many things
#   most important: visualize data with Seaborn
df = pd.read_csv('filename.csv', delimiter=',')

# Or export it in many ways, e.g. a list of tuples
tuples = [tuple(x) for x in df.values]

# or export it as a list of dicts
dicts = df.to_dict().values()

One big advantage is that pandas deals automatically with header rows.

If you haven't heard of Seaborn, I recommend having a look at it.

See also: How do I read and write CSV files with Python?

Pandas #2

import pandas as pd

# Get data - reading the CSV file
import mpu.pd
df = mpu.pd.example_df()

# Convert
dicts = df.to_dict('records')

The content of df is:

     country   population population_time    EUR
0    Germany   82521653.0      2016-12-01   True
1     France   66991000.0      2017-01-01   True
2  Indonesia  255461700.0      2017-01-01  False
3    Ireland    4761865.0             NaT   True
4      Spain   46549045.0      2017-06-01   True
5    Vatican          NaN             NaT   True

The content of dicts is

[{'country': 'Germany', 'population': 82521653.0, 'population_time': Timestamp('2016-12-01 00:00:00'), 'EUR': True},
 {'country': 'France', 'population': 66991000.0, 'population_time': Timestamp('2017-01-01 00:00:00'), 'EUR': True},
 {'country': 'Indonesia', 'population': 255461700.0, 'population_time': Timestamp('2017-01-01 00:00:00'), 'EUR': False},
 {'country': 'Ireland', 'population': 4761865.0, 'population_time': NaT, 'EUR': True},
 {'country': 'Spain', 'population': 46549045.0, 'population_time': Timestamp('2017-06-01 00:00:00'), 'EUR': True},
 {'country': 'Vatican', 'population': nan, 'population_time': NaT, 'EUR': True}]