Ramamurthy attended Tufts University initially as a pre-med major to follow in his parents' footsteps. He was also a member of the Kappa Charge of Theta Delta Chi. He became interested in acting when he took an "Intro to Acting" class during his junior year as part of his graduation requirement. After participating in several plays — including Our Country's Good, he chose to change career goals. He graduated with a bachelor's degree in history and then attended the Webber Douglas Academy of Dramatic Art in London in 1996, graduating in 1998.