Rabbit reticulocyte lysate (RRL) is a mammalian cell-free system for protein production. However, one of the limitations of this system is its low protein yield. Inclusion of recombinant virus proteins and specific viral structures on target mRNA could enhance protein production in RRL.

Why are reticulocyte lysates used for in vitro translation reactions?

Standard in Vitro Translation Procedure Using Rabbit Reticulocyte Lysate or Wheat Germ Extract. Untreated reticulocyte lysate translates endogenous globin mRNA, exogenous RNAs, or both. ... Both lysates are appropriate for the synthesis of larger proteins from either capped or uncapped RNAs (eukaryotic or viral).

What is vitro translation system?

In vitro transcription and in vitro translation replicate the processes of RNA and protein synthesis outside of the cellular environment. ... In vitro translation is a technique that enables researchers to rapidly express and manufacture small amounts of functional proteins for a variety of applications.