Method
- Deparaffinise sections with xylene then take through alcohols to water.
- Oxidise in acidified potassium permanganate for 3 minutes.
- Rinse in distilled water.
- Decolourise with 2% oxalic acid for 1 min.
- Rinse in distilled water.
- Mordant in 4% iron alum for 10 minutes.
- Rinse in distilled water.
What stain is used for Reticulin?
Reticulin fibers are agyrophilic, meaning that these tissue elements will stain black with a silver solution using the aid of a chemical reducer, which brings the silver into a visible form. This silver staining process is known as silver impregnation.
What does reticulin stain for?
In pathology, the reticulin stain, is a popular staining method in histology. It is used to visualize reticular fiber and used extensively in liver histopathology.
