A spectrophotometer is an analytical instrument used to quantitatively measure the transmission or reflection of visible light, UV light or infrared light. ... Applications for specs include measurement of substance concentration such as protein, DNA or RNA, growth of bacterial cells, and enzymatic reactions.

Why do we use spectrophotometry?

Spectrophotometry is a standard and inexpensive technique to measure light absorption or the amount of chemicals in a solution. It uses a light beam which passes through the sample, and each compound in the solution absorbs or transmits light over a certain wavelength.

What is a spectrometer and why is it useful?

A spectrometer is a device for measuring wavelengths of light over a wide range of the electromagnetic spectrum. It is widely used for spectroscopic analysis of sample materials. The incident light from the light source can be transmitted, absorbed or reflected through the sample.