A single low g-force centrifugation step enables mild cell lysis and prevents extensive contact of the nuclei with the cytoplasmic environment. This fast method shows high reproducibility due to the relatively little cell manipulation required by the investigator.

What does centrifugation do to cells?

Centrifugation at different speeds allows the separation of particles into 'fractions', according to how readily they sediment (Box 1). For example, at low centrifugation speeds, large cells can be separated from small cells.

Does centrifuging remove dead cells?

Separation of Dead and Live Cells by Centrifugation

One of the simplest methods of cell debris removal is density-gradient centrifugation. Density-gradient centrifugation harnesses a device called a centrifuge that spins a heterogenous mixture at high speeds.