Agarose gel electrophoresis is most commonly used in the separation of DNA molecules and so is frequently used during DNA manipulation techniques, or studies involving identifying individuals based on their unique DNA sequence.

What is agarose gel used for?

Agarose gel electrophoresis is commonly used to separate DNA fragments following restriction endonuclease digestion or PCR amplification. Fragments are detected by staining the gel with the intercalating dye, ethidium bromide, followed by visualization/photography under ultraviolet light.

Why agarose gel is used for DNA?

Agarose permit the formation of bigger pores and can be used to solve bigger molecule as dna while acrylammide has smaller pores and it is able to solve small molecule as dna fragments or proteins. therefore two molecules with so different size need gels with different resolution.