2-Deoxy- d-glucose is a glucose molecule which has the 2-hydroxyl group replaced by hydrogen, so that it cannot undergo further glycolysis. As such; it acts to competitively inhibit the production of glucose-6-phosphate from glucose at the phosphoglucoisomerase level (step 2 of glycolysis).

How does 2-deoxyglucose affect ATP concentration?

In the presence of 5 mM glucose, incubation of CLL cells with 2DG elicited a 5–10% decrease in ATP at 1 hr and 15–20% reduction in ATP levels at 24 hrs. The effect of 2DG on cell viability was assessed using Alamar Blue.

Does 2-deoxyglucose decrease ATP?

Conversely, 2-deoxyglucose (2DG) blocked glycolysis and partially inhibited glycolytic- dependent oxidative phosphorylation, resulting in a 50% reduction in cellular ATP levels.