Renaturation in molecular biology refers to the reconstruction of a protein or nucleic acid (such as DNA) to their original form especially after denaturation. ... For instance, a heat-denatured DNA can revert to its original form by cooling slowly the two strands and then reform into its original double-stranded helix.

How does DNA denaturation and renaturation happen?

In the process of denaturation, an unwinding of DNA double-strand takes place, leading to two separate single strands on applying heat. Separate single strands rewind on cooling and the process is known as renaturation. ... Renaturation also depends on temperature, pH, length and constituents of the DNA structure.

What is the renaturation of protein?

Renaturation of a protein is the conversion of a denatured protein back into its native 3D structure. Therefore, it involves the reconstruction of a protein molecule after losing its original structure. Renaturation is the inverse process of denaturation.