Darkfield microscopy has many advantages. Its dark background offers a high degree of contrast, making it easy to see samples on difficult backgrounds. This technique is easily accessible since many brightfield lab microscopes can be configured for darkfield illumination.

Why is dark field imaging used?

A dark field microscope is ideal for viewing objects that are unstained, transparent and absorb little or no light. ... You can use dark field to study marine organisms such as algae, plankton, diatoms, insects, fibers, hairs, yeast and protozoa as well as some minerals and crystals, thin polymers and some ceramics.

What are the advantages of dark field microscope?

Improves image contrast without the use of stain, and thus do not kill cells. Direct detection of non-culturable bacteria present in patient samples. Requires no special set up, even a light microscope can be converted to dark field.