The Enzyme-Linked Immunosorbent Assay (ELISA) is a technique used to detect antibodies or infectious agents in a sample. ... For an antigen ELISA, antibodies are bound to a plastic surface, a sample is added and if antigens from the virus we are testing for are present they will stick to the antibodies.
How ELISA works step by step?
ELISA Step-by-step
- Antibody coating. Specific capture antibody is immobilized on high protein-binding plates by overnight incubation. ...
- Protein capture. ...
- Detection antibody. ...
- Streptavidin-enzyme conjugate. ...
- Addition of substrate. ...
- Analysis.
What does an Elisa assay do?
ELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood. An antibody is a protein produced by the body's immune system when it detects harmful substances, called antigens.
