A quantitative measure of broad substrate specificity is yet to be formalized. ... A trait related to broad substrate specificity is promiscuity, which is defined as the catalysis of reactions distinct from the one the protein has evolved to perform, but using the same active site scaffold [15]–[18].

What does broad substrate specificity mean?

The existence of promiscuous enzymes and enzymes with broad substrate specificity suggests that active sites possess a degree of flexibility and that it is this flexibility that facilitates evolution. Enzymes are malleable not immutable – they possess a degree of plasticity.

Which of the following is an example for a broad substrate specificity?

Explanation: The specificity in which the enzyme acts on more than one structurally related substrates is referred to as broad substrate specificity. The example for this type of specificity is hexokinase phosphorylating one or more kind of hexoses which include glucose, mannose fructose etc.