Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin. Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of E. coli and other microorganisms.

How do you prepare tryptone?

Tryptone Broth is used for the detection of indole production by coliforms. Suspend 15 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

What is tryptone powder?

Product Description: Tryptone Powder contains a mixture of amino acids and is an excellent nutrient for use in culture media for producing antibiotics, toxins, enzymes, and other biological products. This product is widely used in the pharmaceutical and veterinary industries and in diagnostic culture media.