Solubility does not affect chromatography; differences in intermolecular forces affect chromatography.

How does solubility affect chromatography?

Higher the solubility of the constituents in the mobile phase, faster will be the mobility rate. Hence, the solubility of the components of the mixture affects the process of chromatography as higher the solubility in the mobile phase, faster the components will elute out of the chromatography stationary phase.

What are the factors affecting chromatography?

Retention factor values in thin layer chromatography are affected by the absorbent, the solvent, the chromatography plate itself, application technique and the temperature of the solvent and plate.