It is best to start decalcification at the beginning of the week and never the weekend in order to maintain continual observation of the tissue. 1. Remove excess tissue from around the bone if the tissue is not needed. This will allow for better penetration of decalcifying solution into the tissue.

Between what processes should decalcification be done?

Decalcification is usually carried out between the fixation and processing steps. Bone must obviously be processed in this way, but other tissues may also contain calcified areas. A variety of agents or techniques have been developed to decalcify tissues, each with advantages and disadvantages.

Why is decalcification done after fixation?

Fixation of bone

In order to protect the cellular and fibrous elements of bone from damage caused by the acids used as decalcifying agents, it is particularly important to thoroughly fix these specimens prior to decalcification.