In a pour plate, a small amount of inoculum from a broth culture is added by pipette to the centre of a Petri dish. Cooled, but still molten, agar medium in a test tube or bottle is then poured into the Petri dish.
What is the pour plate method quizlet?
The pour plate method involves diluting one loopful of bacterial culture into a series of test tubes containing. Melted nutrient agar.
What are the steps in the streak plate method?
Procedure for Streaking a Plate for Isolation:
- Flame the loop and wire and streak a loopful of broth as at A in the diagram.
- Reflame the loop and cool it.
- Streak as at B to spread the original inoculum over more of the agar.
- Reflame the loop and cool it.
- Streak as at C.
- Reflame the loop and cool it.
- Streak as at D.
