Embryonated chicken eggs are inoculated by the allantoic route at approximately the middle of the 21-day embryonation period, at 8–10 days of embryonation; they are inoculated by the amniotic route late in the incubation period, at 14–16 days of embryonation.

What are the routes of egg inoculation?

The different routes of inoculation in mice are intracerebral, subcutaneous, intraperitoneal or intranasal. After the animal is inoculated with the virus suspension, the animal is observed for signs of disease, visible lesions or is killed so that infected tissues can be examined for virus.

What is embryo inoculation in technique?

Viruses are inoculated into chick embryo of 7-12 days old. For inoculation, eggs are first prepared for cultivation, the shell surface is first disinfected with iodine and penetrated with a small sterile drill. After inoculation, the opening is sealed with gelatin or paraffin and incubated at 36°c for 2-3 days.