Plasmids are autonomously replicating circular extra-chromosomal DNA. They are the standard cloning vectors and the ones most commonly used. Most general plasmids may be used to clone DNA insert of up to 15 kb in size. One of the earliest commonly used cloning vectors is the pBR322 plasmid.
Which features are desirable in a plasmid cloning vector?
Desirable properties of plasmids
- It should be small. The aim of most cloning experiments is to isolate the passenger DNA. ...
- Its DNA sequence should be known. ...
- Plasmid vectors should contain a selectable marker that allows cells containing the plasmid to be isolated.
How are plasmids used as cloning vectors?
Scientists have taken advantage of plasmids to use them as tools to clone, transfer, and manipulate genes. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
