It's best to collect sputum specimens first thing in the morning, when you get up. Collect specimens only at that time unless instructed to do otherwise by hospital personnel or your physician.

When is the best time to get a sputum sample?

Best time of day to collect sputum is when you first wake. Do not eat, drink or smoke before coughing up sputum from the lungs. Rinse (do not swallow) the mouth with water before sputum is collected to minimize residual food particles, mouthwash, or oral drugs that might contaminate the specimen.

How fresh does a sputum sample need to be?

The sample can be refrigerated for up to 24 hours if needed. Don't freeze it or store it at room temperature. If you can't cough up sputum, try breathing the steam from boiling water or take a hot, steamy shower. The sputum must come from deep inside your lungs for the test to be accurate.