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Yes, it does!
How do I enable AppLocker in Windows 10 pro?
Go to Security Settings > Application Control Policies > AppLocker, and select Configure rule enforcement. Check Configured under Executable rules, and then click OK.
What versions of Windows support AppLocker?
AppLocker is available in all editions of Windows Server 2008 R2 and in Windows 7 Ultimate and Windows 7 Enterprise. Windows 7 Professional can be used to create AppLocker rules.