This compound works by interrupting protein synthesis in both prokaryotic and eukaryotic cells. This causes cells to die, unless they have a gene for resistance, allowing them to survive exposure to the antibiotic.

How long does puromycin selection take?

Puromycin selection requires a minimum of 48 hours. Optimum effectiveness should be reached within 3-10 days. Assay transfected cells.

How does puromycin resistance gene work?

Cell culture

It is toxic to prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain. ... Puromycin acts quickly and can kill up to 99% of nonresistant cells within 2 days.