Does Centrifuging Kill Cells?

Does Centrifuging Kill Cells?

Centrifugation in essence involves compacting bacteria into a pellet, causing collisions against each other that result in shear forces on the bacterial cell surface, which may easily lead to cell surface damage with a potential effect on the outcome of surface-sensitive experiments.

Does centrifuge kill cells?

It is well known that centrifugation kills cells. I spin my cells after trypsinisation at 100g. What you propose is to spin them at 32 times that normal speed. You will capture your dead cells but you will also cause more cells to apoptose and die.

What happens when you centrifuge cells too fast?

Spinning too fast can cause a "smear" of cells up the wall of the tube that you may be missing when resuspending the cells.

Chloe Bennett
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Chloe Bennett

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