Bulked segregant analysis (BSA) is a technique used to identify genetic markers associated with a mutant phenotype. This allows geneticists to discover genes conferring disease resistance or susceptibility. This technique involves forming two groups that display opposing phenotypes for a trait of interest.

What is the usefulness of bulked segregant analysis strategy in major gene mapping?

Bulked segregant analysis (BSA) has been used to map many agronomical traits in several crops, including resistance genes to fungal pathogens. Using BSA in diploid progenies, it was proposed that the inheritance of ABS resistance in citrus is controlled by a single recessive allele (Dalkilic et al., 2005).

What is bulk in genetics?

There are two ways to bulk sampled individuals. Tissues sampled from the phenotypic extremes are pooled first, and then, a single DNA/RNA/protein isolation is performed; or DNA/RNA/protein is isolated first from each extreme individual, and then, an equal amount of the extraction from each individual is bulked.