A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. Western blots can also be used to evaluate the size of a protein of interest, and to measure the amount of protein expression. ...

Why use Western blot instead of Elisa?

Western Blotting is the most common method of testing to confirm positive results from ELISA test. ... One advantage of Western Blotting is that it's less likely to give false positive results as it can effectively distinguish between HIV antibodies and other antibodies.

Why the name is Western blotting?

Western blotting was named in a nod to a tradition that had been inadvertently started when Edwin Southern penned his new invention 'the Southern blot'. What is western blotting? In 1975, Southern invented a new method that enabled analysis of DNA identity, size and abundance.