The antibiotic resistance gene allows for selection in bacteria. However, many plasmids also have selectable markers for use in other cell types. A short single-stranded DNA sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited for sequence verification of plasmids.
What is selectable marker in plasmid?
A selectable marker is a gene introduced into a cell, especially a bacterium or to cells in culture, that confers a trait suitable for artificial selection.
What is the role of selectable marker in a plasmid?
Complete answer: -Selectable marker genes are found in plasmids and they help in the selection of the transformants from the non-transformants. ... -Selectable marker genes may give resistance to antibiotics especially the ampicillin-resistant and the tetracycline-resistant sites which are seen in the plasmid pBR322.