For removal of platelets, resuspend the cell pellet in 50 mL of buffer and centrifuge at 200×g for 10–15 minutes at 20 °C. Carefully remove the supernatant completely. ▲ Note: This step will increase the purity of the target cells in the subsequent MACS® Cell Separation.

Are platelets in PBMCs?

Peripheral blood mononuclear cells (PBMCs) that are prepared based on a density gradient method are contaminated by platelets. The platelet contamination can contribute to overestimation of mtDNA measurements. Despite this fact, PBMCs are still used without removing platelets in many studies.

How is platelet separation from blood?

Platelets are made in our bone marrow. Platelets can be prepared by using a centrifuge to separate the platelet-rich plasma from donated whole blood. Platelets from several different donors are then combined to make one tranfusable unit.