Ringer's saline solution was invented in the early 1880s by Sydney Ringer, a British physician and physiologist. Ringer was studying the beating of an isolated frog heart outside of the body. He hoped to identify the substances in blood that would allow the isolated heart to beat normally for a time.
Why is it called Ringer's solution?
Ringer's solution is named after Sydney Ringer, who in 1882–1885 determined that a solution perfusing a frog's heart must contain sodium, potassium and calcium salts in a definite proportion if the heart is to be kept beating for long.
How do you make Ringer's solution?
- In a beaker add 500 ml of distilled water.
- Dissolve the reagents into the beaker with distilled water.
- Add water to bring the final volume to 1 L.
- Adjust the pH to 7.3-7.4.
- Filter the solution through a 0.22-μm filter.
- Autoclave Ringer's solution prior to use.
