Proteins produced from such DNA templates are called recombinant proteins. Traditional strategies for recombinant protein expression involve transfecting cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein.

What expression systems are used for recombinant protein production?

The baculovirus expression vector system (BEVS) is used for the production of recombinant proteins in insect cells. The insect cells are grown to the desired cell density and then infected with a recombinant baculovirus containing the gene of interest (Owczarek et al., 2019).

What are the common promoters used for the recombinant protein expression in E coli?

The pET expression system featuring the T7 promoter is by far the most widely used system for heterogeneous expression in E. coli [57]. T7 promoter activity is strong, and a recombinant protein can accumulate to up to 50% of total cellular proteins [58].