Primer dimers form when two primers bind to one another, instead of to the template DNA, due to regions of primer complementarity.
Why do primer dimers occur in PCR?
A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As a result, the DNA polymerase amplifies the PD, leading to competition for PCR reagents, thus potentially inhibiting amplification of the DNA sequence targeted for PCR amplification. ...
How do you prevent primer dimers from forming?
i suggest one (or more) of the following solutions:
- increase the annealing temperature.
- increase time\ temperature of template denaturation.
- decrease primers concentration(10 pmol will be OK)
- use a PCR enhancer such as DMSO.
- Check out your template. ...
- use high quality Tag.
