Supply-demand cycle:
Milk production is a demand-supply process. More nursing/pumping results in a greater milk supply. If you consistently decrease nursing or pumping for several days, your overall milk supply will decrease and you can expect to see a decrease in pumped amounts.
Does pumping milk reduce supply?
Actually, no — it's the opposite. Waiting too long to nurse or pump can slowly reduce your milk supply. The more you delay nursing or pumping, the less milk your body will produce because the overfilled breast sends the signal that you must need less milk.
Does expressing milk increase supply?
If you have to spend time apart from your baby, and you're unable to breastfeed, this will impact the amount of breastmilk you produce. Regularly expressing your milk will help maintain your milk supply.