Lowenstein-Jensen (LJ) is the selective medium which is used for the cultivation and isolation of Mycobacterium species. It was developed by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria. ... This formulation also encourages the earliest possible growth of mycobacteria.
What is Lowenstein Jensen medium used for?
Löwenstein–Jensen medium, more commonly known as LJ medium, is a growth medium specially used for culture of Mycobacterium species, notably Mycobacterium tuberculosis. When grown on LJ medium, M. tuberculosis appears as brown, granular colonies (sometimes called "buff, rough and tough").
What medium is used for Mycobacterium tuberculosis?
The only media that allow abundant growth of M. tuberculosis are egg-enriched media with glycerol and asparagine (viz., Lowenstein- Jensen) or agar based media supplemented with bovine albumin (viz., Middlebrook, 7H10 or 7H11).