Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.

How do you use cycloheximide?

Directions for Use: Cycloheximide is supplied as a lyophilized powder. For a 10 mg/ml stock, carefully weigh out and reconstitute 50 mg in 5 ml DMSO or EtOH. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µg/ml for 4-24 hours.

Is cycloheximide an antifungal?

Cycloheximide is an antibiotic with significant antifungal properties. It is produced by some streptomycin-producing strains of Streptomyces griseus and acts by inhibiting protein synthesis. ... Thus, this drug can be a good antifungal agent in the Optisol medium for corneal preservation.