A competitive binding assay typically measures the binding of a labeled ligand to a target protein in the presence of a second, competing but unlabeled ligand. This assay can be used to assess qualitative binding information as well as relative affinities of two or more molecules for one target.

What is the definition of competitive binding?

Competitive binding refers instead to the experimental situation in which both the drug and the natural ligand are added to the receptor without preincubation.

What are ligand binding assays used for?

A ligand binding assay is used to quantify biotherapeutics and biomarkers and to detect anti-drug antibodies in biological matrices by measuring the interaction between two molecules or the binding of molecules to antibodies, receptors, and other large complex molecules.