Every chromosome has at least two microtubules extending from its kinetochore — with at least one microtubule connected to each pole. ... Metaphase leads to anaphase, during which each chromosome's sister chromatids separate and move to opposite poles of the cell.

What happens to the chromosomes during metaphase?

During metaphase, the cell's chromosomes align themselves in the middle of the cell through a type of cellular "tug of war." The chromosomes, which have been replicated and remain joined at a central point called the centromere, are called sister chromatids.

Do chromosomes separate from each other during metaphase?

The chromosomes begin moving toward the equator of the cell. During metaphase II, the centromeres of the paired chromatids align along the equatorial plate in both cells. Then in anaphase II, the chromosomes separate at the centromeres. The spindle fibers pull the separated chromosomes toward each pole of the cell.