gBlocks™ Gene Fragments are double-stranded DNA fragments of 125–3000 bp in length. They are the industry standard for double-stranded gene fragments, designed for affordable and easy gene construction or modification, applications such as antibody research and CRISPR-mediated genome editing, qPCR standards, and more.

What are gBlocks used for?

gBlocks Gene Fragments have been used in a wide range of applications including CRISPR-mediated genome editing, antibody research, codon optimization, mutagenesis, and aptamer expression. They can also be used for generating qPCR standards.

Can you PCR a Gblock?

If you must amplify your gBlocks fragment, use the minimum amount of starting template required by your PCR kit protocol, limit the cycle number to 10–12, and use a high-fidelity polymerase.

What is a gene fragment?

Gene fragments are pieces of genes containing only the exons (those parts of the gene which actually encode the protein sequence).

What is IDTE buffer?

IDTE (10 mM Tris, 0.1 mM EDTA) is our recommended solution for resuspending and storing single-stranded DNA and RNA oligos. It has been shown to offer the most stability for the longest duration when compared to oligos stored dry or in water. IDTE is available at pH 7.5 or pH 8.0.

What is the meaning of DNA synthesis?

DNA synthesis is the process whereby deoxynucleic acids (adenine, thymine, cytosine, and guanine) are linked together to form DNA.

What are DNA fragments called?

DNA fragments cut by restriction enzymes are called restriction fragments. They can be separated by gel electrophoresis. Further reading: Plasmid.

What are DNA fragments made of?

DNA fragments are composed of molecules called nucleotides. Each nucleotide is made up of a sugar group, a phosphate group, and a nitrogen base.

What causes DNA fragmentation?

DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode. Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.

What is duplex oligo?

Some types of reactions demand that a double-chain DNA (oligo DUPLEX) be produced out of complementary single-chain oligonucleotides (ssDNA). There is a high risk of emergence of undesirable secondary structures, e.g. hairpin loops, when complementary ssDNAs are simply mixed at laboratory temperature.

What is 1X TE buffer?

TE Buffer, 1X, Molecular Grade (pH 8.0), is a buffer composed of 10mM Tris-HCl containing 1mM EDTA•Na2. Properties: pH at 25°C: 7.9–8.1.

What temperature should primers be stored at?

Primers, when suspended, should be stored in a freezer at -20C.