In numerous tests carried out by independent, licensed testing laboratories, SYBR Safe DNA Gel Stain showed little or no genotoxicity and no acute toxicity.
Is SYBR Safe a carcinogen?
SYBR Safe® Alternative to Ethidium Bromide
1 Whereas EtBr tested positive in the Syrian hamster embryo (SHE) cell assay, SYBR Safe® tested negative, indicating that SYBR Safe® is not suspected to be carcinogenic.
How much SYBR is safe for DNA gel stain?
1.1 Soak the gel in SYBR® Safe stain.
If using SYBR® Safe gel stain concentrate, dilute 10,000X in TAE or TBE buffer (as appropriate) prior to use. Place the gel in a plastic container, such as a pipet-tip box lid or a household food-storage container.
Can SYBR Safe be used for RNA?
Yes, the stain is also suitable for staining RNA in gels. Bound to nucleic acids, SYBR Safe stain has fluorescence excitation maxima at 280 and 502 nm, and an emission maximum at 530 nm.
Is SYBR Safe safer than ethidium bromide?
SYBR® Safe
It is marketed as being less harmful than ethidium bromide, but this is debatable. Its major advantage is that it is as sensitive as ethidium bromide but does not require UV light for visualization. Toxicity: Documented as less mutagenic that ethidium bromide, but its acute toxicity is higher.
Is SYBR Safe mutagenic?
SYBR® Safe DNA Gel Stain was weakly mutagenic only with S9 metabolic activation in the standard Ames bacterial mutation assay. Compared to ethidium bromide, SYBR® Safe DNA Gel Stain caused fewer mutations, as measured in several different strains of Salmonella typhimurium.
How does SYBR Safe Work?
However, since DNA is clear and colorless, the bands cannot be seen with the naked eye. SYBR Safe® is a fluorescent DNA stain that binds specifically to the DNA double helix. When excited with UV or blue light, any SYBR Safe® that is bound to DNA fluoresces with a bright green color.
What is the purpose of SYBR Safe?
Invitrogen SYBR Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. SYBR Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can be used with either blue-light or UV excitation.
Is SYBR Green toxic?
Thus, SYBR Gold and SYBR Green II do not show mutagenicity in our tests, even at toxic doses, and these DNA stains represent safer alternatives to ethidium bromide for nucleic acid visualization.
How do you add a SYBR Safe to gel?
Add sufficient SYBR™ Safe DNA gel stain to cover the gel. A 50 mL volume is sufficient for staining most standard minigels. To stain larger gels, increase the volume of staining solution in proportion to the increased gel volume, and ensure that the gel is fully immersed during staining. 1.2 Incubate for 30 minutes.
Why is SYBR Safe added to agarose gel?
Agarose gel electrophoresis is used to separate mixtures of DNA fragments into discrete bands according to their size. However, since DNA is clear and colorless, the bands cannot be seen with the naked eye. SYBR Safe® is a fluorescent DNA stain that binds specifically to the DNA double helix.
Does SYBR Safe stain single stranded DNA?
SYBR™ Gold stain is a proprietary unsymmetrical cyanine dye that exhibits >1000-fold fluorescence enhancement upon binding to nucleic acids and has a high quantum yield (∼0.6) upon binding to double- or single-stranded DNA or to RNA1.
Does SYBR Green bind RNA?
SYBR Green II RNA gel stain is a sensitive nucleic acid gel stain that has bright fluorescence when bound to RNA and low background in gels, making it ideal for use with either formaldehyde/agarose or polyacrylamide gels using laser scanners or standard UV transilluminators.
Can SYBR Green Stain RNA?
Without any washing or destaining steps, SYBR Green II can detect as little as 1 ng of RNA per band in agarose/formaldehyde gels or polyacrylamide/urea gels using 254 nm epi-illumination, and ∼4 ng of RNA per band using 300 nm transillumination.
Is SYBR green light sensitive?
SYBR Green I dye (present in the master mix) is light-sensitive; solutions containing the master mix should be protected from light whenever possible. Note Total reaction volumes of 50 μl may also be used.
How long does SYBR safe last?
SYBR safe has to be mixed 1/10,000 in the same buffer in which you made your gel and the gel can be soaked in it before or after it is run. If you are resolving small fragments, before is recommended so there is no time for diffusion after. The bath should be kept protected from the dark and is good for about 2 weeks.
Why is SYBR Green Safe?
SYBR Safe is a cyanine dye used as a nucleic acid stain in molecular biology. SYBR Safe is one of a number of SYBR dyes made by the Life Technologies Corporation. SYBR Safe binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 509 nm) and emits green light (λmax = 524 nm).
Does SYBR safe expire?
SYBR Safe’s shelf life is 1 year.
