Restriction fragment length polymorphisms, or RFLPs, are differences among individuals in the lengths of DNA fragments cut by enzymes. … RFLP analysis can be used as a form of genetic testing to observe whether an individual carries a mutant gene for a disease that runs in his or her family.
How is RFLP used to identify a person?
Once a disease gene was localized, RFLP analysis of other families could reveal who was at risk for the disease, or who was likely to be a carrier of the mutant genes. RFLP test is used in identification and differentiation of organisms by analyzing unique patterns in genome.
How do you do RFLP?
- DNA Extraction. To begin with, DNA is extracted from blood, saliva or other samples and purified.
- DNA Fragmentation. The purified DNA is digested using restriction endonucleases. …
- Gel Electrophoresis. …
- Visualization of Bands.
