The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa. GFPuv is the Aequorea sequence with three aa substitutions; Phe to Ser at # 99, Met to Thr at # 153, and Val to Ala at # 163. …

What is GFPuv used for?

By the convenient measure of fluorescence intensity, GFPuv can be used as an indicator to report the extent of denaturation rates of other proteins in glucose solutions.

Why is E coli used for GFP?

coli was used as the target protein for fusion with GFP because it is a well-characterized exported protein normally localized to the periplasmic space. Gene fusions between gfp and malE were made as described in Materials and Methods, yielding the constructs shown in Fig.