pUC8 is a derivative of plasmid pBR322 and filamentous phage M13. Plasmid pUC8:15 bears a 2.3-kb Vitreoscilla chromosomal fragment (Hind IIIHind III) containing the Vitreoscilla hemoglobin gene (vgb).

Is pUC8 a cloning vector?

Cloning Vector: A cloning vector is defined as a vector used for replication of a cloned DNA fragment in a host cell. These vectors are frequently engineered to contain “ori” – origin of replication sites particular to the host organism. Examples of commonly used cloning vectors are: pUC18, pUC19, pBluescript vectors.

Why is pUC19 a good cloning vector?

pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. … NEB offers a selection of common cloning plasmids and DNAs for use as substrates.