Negative selection (immunology), in which B-cells and T-cells that recognize MHC molecules bound to peptides of self-origin, or just MHC molecules with high affinity are deleted from the repertoire of immune cells.

What is negative selection cells?

Negative selection is when several cell types are removed, leaving the cell type of interest untouched. Similar to positive selection methods, cells are labeled with antibodies that target specific cell surface markers or populations.

How does negative selection occur?

Negative selection occurs when double positive T cells bind to bone-marrow derived APC (macrophages and dendritic cells) expressing Class I or Class II MHC plus self peptides with a high enough affinity to receive an apoptosis signal.