Cuvettes are designed to hold samples for spectroscopic measurement, where a beam of light is passed through the sample within the cuvette to measure the absorbance, transmittance, fluorescence intensity, fluorescence polarization, or fluorescence lifetime of the sample.

Do cuvettes absorb light?

The samples are prepared in cuvettes that are made using specialized plastics or quartz so that they do not absorb any light and will not affect our measurements. … Compared to the amount of light entering the sample, the amount that exits is measured as a percentage of the light transmitted.

How does cuvette size affect absorbance?

The absorbance is directly proportional to the concentration (c) of the solution of the sample used in the experiment. The absorbance is directly proportional to the length of the light path (l), which is equal to the width of the cuvette.