The catalase test is a particularly important test used to determine whether a gram-positive cocci is a staphylococci or a streptococci. Catalase is an enzyme that converts hydrogen peroxide to water and oxygen gas. The test is easy to perform; bacteria are simply mixed with H2O2.

What does a catalase test indicate?

This test is used to identify organisms that produce the enzyme, catalase. This enzyme detoxifies hydrogen peroxide by breaking it down into water and oxygen gas. The bubbles resulting from production of oxygen gas clearly indicate a catalase positive result.

What is the purpose of a catalase test in microbiology?

The catalase test facilitates the detection of the enzyme catalase in bacteria. It is essential for differentiating catalase- positive Micrococcaceae from catalase-negative Streptococcaceae.