A microtome is used to make thin slices of tissue (usually 4 μm but can be 2–10 μm). The cut tissue is floated over a water bath, in order to eliminate wrinkles and distortion in the tissue, and picked up on a slide.

What is working principle of microtome?

The vibrating microtome operates by cutting using a vibrating blade, allowing the resultant cut to be made with less pressure than would be required for a stationary blade. The vibrating microtome is usually used for difficult biological samples.

How is the microtome's knife sharpen?

Microtome knife sharpeners are safe and easy to use, provide efficient sharpening of microtome knife edges. Honing procedure of knife is done through glass plates, copper and bronze plates. Honing refers to grinding the cutting edge of a knife on the hard abrasive surface to sharpen the knife.