Dilution is the process of decreasing the concentration of a solute in a solution, usually simply by mixing with more solvent like adding more water to the solution. … The resulting solution is thoroughly mixed so as to ensure that all parts of the solution are identical.

What is macro broth dilution method?

Definition. A method to determine the minimum inhibitory concentration (MIC) of antibiotics in which growth media is pipetted into a multi-well plate. Each well contains a known concentration of drug which is inoculated with a known quantity of bacteria.

How do you dilute antibiotics?

Working dilution: 1/1000, so you have to put 500 µL stock solution in 500 mL of your medium, for example. Your final working concentration is now 100 µg/mL. Working dilution: 1/100, so you have to put 5 mL stock solution in 500 mL of your medium, for example. Your final working concentration here is also 100 µg/mL.