The samples are prepared in cuvettes that are made using specialized plastics or quartz so that they do not absorb any light and will not affect our measurements. … Compared to the amount of light entering the sample, the amount that exits is measured as a percentage of the light transmitted.

Where are cuvettes used?

Cuvettes are a small plastic or glass tubes of circular or square cross section, usually sealed at one end. They are used to hold samples for spectroscopic analysis. Cuvettes may be open to the atmosphere at the top or have an additional cap to seal them shut.

Why are cuvettes used?

Cuvettes are designed to hold samples for spectroscopic measurement, where a beam of light is passed through the sample within the cuvette to measure the absorbance, transmittance, fluorescence intensity, fluorescence polarization, or fluorescence lifetime of the sample.